Chronic measurement of left ventricular pressure in freely moving rats

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Authors
Stehlin, Ellyce
Malpas, Simon C.
Budgett, David M.
Barrett, Carolyn J.
McCormick, Daniel
Whalley, Gillian
Fu, Fumin
Beil, Michael
Rigel, Dean F.
Guild, Sarah-Jane
Author ORCID Profiles (clickable)
Degree
Grantor
Date
2013-10-08
Supervisors
Type
Journal Article
Ngā Upoko Tukutuku (Māori subject headings)
Keyword
telemetry
solid-state pressure
inductive power
dP/dt
rats
ANZSRC Field of Research Code (2020)
Citation
Stehlin, E., Malpas, S., Budgett, D., Barrett, C., Mccormick, D., Whalley, G., Fu, F., Bell, M., RIgel, D.F.and Guild, S. (2013). Chronic measurement of left ventricular pressure in freely moving rats. Journal of Applied Physiology (Bethesda, Md. : 1985), 115(11), 1672-82.
Abstract
Measurements of left ventricular pressure (LVP) in conscious freely moving animals are uncommon yet could offer considerable opportunity for understanding cardiovascular disease progression and treatment. The aim of this study was to develop surgical methods and validate the measurements of a new high-fidelity solid-state pressure sensor telemetry device for chronically measuring LVP and dP/dt in rats. The pressure sensor catheter tip (2-Fr) was inserted into the left ventricular chamber through the apex of the heart and the telemeter body implanted in the abdomen. Data was measured up to 85 days after implant. The average daytime dP/dt max was 9444 ±363 mmHg/s, ranging from 7870 to 10558 mmHg/s (n=7). A circadian variation in dP/dt max and heart rate (HR) was observed with an average increase during the night phase in dP/dt max of 918 ±84 mmHg/s and in HR of 38 ±3 bpm. The β-adrenergic-agonist isoproterenol, β1-adrenergic agonist dobutamine, Ca2+ channel blocker verapamil and the calcium sensitizer levosimendan were administered throughout the implant period, inducing dose dependent time course changes and absolute changes in dP/dt max of -6000 to +13,000 mmHg/s . The surgical methods and new technologies demonstrated long term stability, sensitivity to circadian variation and the ability to measure large drug induced changes, validating this new solution for chronic measurement of LVP in conscious rats.
Publisher
American Physiological Society
Link to ePress publication
DOI
doi:10.1152/japplphysiol.00683.2013
Copyright holder
American Physiological Society
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8750-7587/13 Copyright © 2013 the American Physiological Society
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