Environmental bio-monitoring with high-throughput sequencing
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Other Title
Authors
Wang, Jing
McLenachan, P.A.
Biggs, Patrick J.
Winder, Linton
Schoenfeld, Barbara I.K.
Narayan, Vinay V.
Phiri, Bernard J.
Lockhart, Peter
McLenachan, P.A.
Biggs, Patrick J.
Winder, Linton
Schoenfeld, Barbara I.K.
Narayan, Vinay V.
Phiri, Bernard J.
Lockhart, Peter
Author ORCID Profiles (clickable)
Degree
Grantor
Date
2013-05-15
Supervisors
Type
Journal Article
Ngā Upoko Tukutuku (Māori subject headings)
Keyword
metagenomics
environmental bio-monitoring
high-throughput sequencing
environmental bio-monitoring
high-throughput sequencing
ANZSRC Field of Research Code (2020)
Citation
Wang, J., McLenachan, P.J., Biggs, P.J., Winder, L. H., Schoenfeld, B.I.K., Narayan, V.V., Phiri, B.J., and Lockhart, P.J. (2013). Environmental bio-monitoring with high-throughput sequencing. Briefings in Bioinformatics.
Abstract
There is much interest in using high-throughput DNA sequencing methodology to monitor microorganisms, complex plant and animal communities. However, there are experimental and analytical issues to consider before applying a sequencing technology, which was originally developed for genome projects, to ecological projects. Many of these issues have been highlighted by recent microbial studies. Understanding how high-throughput sequencing is best implemented is important for the interpretation of recent results and the success of future applications.
Addressing complex biological questions with metagenomics requires the interaction of researchers who bring different skill sets to problem solving. Educators can help by nurturing a collaborative interdisciplinary approach to genome science, which is essential for effective problem solving. Educators are in a position to help students, teachers, the public and policy makers interpret the new knowledge that metagenomics brings. To do this, they need to understand, not only the excitement of the science but also the pitfalls and shortcomings of methodology and research designs. We review these issues and some of the research directions that are helping to move the field forward.
Publisher
Oxford University Press
Permanent link
Link to ePress publication
DOI
doi: 10.1093/bib/bbt032
Copyright holder
The Authors
Copyright notice
© The Author 2013. Published by Oxford University Press. For Permissions, please email: journals.permissions@oup.com