DNA extraction and amplification of faecal samples from the White Fronted Terns (Sternula nereis). Report prepared for North New Zealand Seabird Trust. 6 p.
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Authors
Doyle, Erin
Adams, Nigel
Adams, Nigel
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Date
2019
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Other
Ngā Upoko Tukutuku (Māori subject headings)
Keyword
New Zealand birds
White Fronted Tern (Sternula nereis)
Sternula nereis
faecal samples
DNA barcoding
DNA extraction
White Fronted Tern (Sternula nereis)
Sternula nereis
faecal samples
DNA barcoding
DNA extraction
ANZSRC Field of Research Code (2020)
Citation
Doyle, E., & Adams, N. (2019). DNA extraction and amplification of faecal samples from the White Fronted Terns (Sternula nereis). Report prepared for North New Zealand Seabird Trust. 6 p. Auckland.
Abstract
This report describes the success of DNA extraction and amplification of fresh faecal samples collected from White Fronted Tern Sterna striata in the field. Sample handling involved immediate placement in ethanol field followed by freezing at -20oC a few hours later on the day of collection.. DNA was extracted from 10 faecal samples collected from birds (Sternula nereis). PCR using primers targeting Chordata was then used to selectively amplify short sections of the 16S gene which can be used for prey identification . PCR products were run on an agarose gel, as a success/fail test for amplification. DNA was successfully amplified in four of the ten samples. Attempts to amplify DNA using primers targeting Malacostraca and a second pair of PCR primers, targeting a 180-270bp section of the 16S gene of multiple taxa, were unsuccessful.
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Unitec Institute of Technology. Applied Molecular Solutions
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