Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae)
Bulgarella, M.; Biggs, Patrick J.; de Lange, Peter; Shepherd, L.D.
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Citation:Bulgarella, M., Biggs, P., de Lange, P.J., & Shepherd, L. (2017). Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae). Applications of Plant Sciences, 5, pp.1700041. doi:doi.org/10.3732/apps.1700041
Permanent link to Research Bank record:https://hdl.handle.net/10652/4119
• PREMISE OF THE STUDY: Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species. • METHODS AND RESULTS: Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum. • CONCLUSIONS: These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.