Chronic measurement of left ventricular pressure in freely moving rats

Abstract

Measurements of left ventricular pressure (LVP) in conscious freely moving animals are uncommon yet could offer considerable opportunity for understanding cardiovascular disease progression and treatment. The aim of this study was to develop surgical methods and validate the measurements of a new high-fidelity solid-state pressure sensor telemetry device for chronically measuring LVP and dP/dt in rats. The pressure sensor catheter tip (2-Fr) was inserted into the left ventricular chamber through the apex of the heart and the telemeter body implanted in the abdomen. Data was measured up to 85 days after implant. The average daytime dP/dt max was 9444 ±363 mmHg/s, ranging from 7870 to 10558 mmHg/s (n=7). A circadian variation in dP/dt max and heart rate (HR) was observed with an average increase during the night phase in dP/dt max of 918 ±84 mmHg/s and in HR of 38 ±3 bpm. The β-adrenergic-agonist isoproterenol, β1-adrenergic agonist dobutamine, Ca2+ channel blocker verapamil and the calcium sensitizer levosimendan were administered throughout the implant period, inducing dose dependent time course changes and absolute changes in dP/dt max of -6000 to +13,000 mmHg/s . The surgical methods and new technologies demonstrated long term stability, sensitivity to circadian variation and the ability to measure large drug induced changes, validating this new solution for chronic measurement of LVP in conscious rats.